Here we report that an engineered microbial cytochrome P450 BM-3 (CYP102A subfamily) efficiently catalyzes the alpha-hydroxylation of phenylacetic acid esters. This P450 BM-3 variant also produces the authentic human metabolite of buspirone, R-6-hydroxybuspirone, with 99.5% ee.

Engineered microbial cytochrome P450 BM-3 efficiently catalyzes alpha-hydroxylation of phenylacetic acid esters and produces the authentic human metabolite of buspirone, R-6-hydroxybuspirone, with 99.5% ee.

Paper

Enantioselective alpha-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3.

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