Paper
Antioxidative and Acute Anti-inflammatory Effects of Spirodela polyrhiza Extract in Human HaCaT Keratinocytes and NC/Nga Mice
Published Dec 1, 2009 · J. Mok, Seung-Il Jeong, S. Jang
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Abstract
Oxidative stress is involved in many human diseases in the process of inflammation in various tissues including the skin. Therefore, the present study was undertaken to investigate the antioxidative and anti-inflammatory activities of the extracts of Spirodela polyrhiza in Human HaCaT Keratinocytes and NC/Nga mice. Exposure of human HaCaT keratinocytes to methanol extract of Spirodela polyrhiza (ESP) at 50 and 400 ㎍/㎖ showed significant protective effect against 500 ㎛ of hydrogen peroxide (H2O2). ESP not only protected cell survival from H2O2-induced toxicity, but also inhibited the H2O2-induced leakage of lactate dehydrogenase (LDH) enzyme release and DNA fragmentation significantly. It was also found that ESP showed scavenging activities of l,1-diphenyl-2-picrylhydrazyl (DPPH)-induced radicals and superoxide radicals in the xanthine/xanthine oxidase system, respectively. Interestingly, pretreatment with ESP significantly suppressed lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production through the inhibition of inducible NO synthase (iNOS) expression. Furthermore, topically applied ESP dose-dependently inhibited 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ear edema in NC/Nga mice. Consistent with its antioxidative properties in vitro, our data suggest that the therapeutic potential of ESP for acute skin inflammation that may involve oxidative tissue damage.
Non-RCT
Animal StudySpirodela polyrhiza extract shows antioxidative properties and shows potential therapeutic potential for acute skin inflammation involving oxidative tissue damage.
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