Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.

doi:10.1529/BIOPHYSJ.105.064816

Paper

Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.

Published Jul 1, 2005 · Lei Jin, A. Millard, J. Wuskell

Biophysical journal

Q1 SJR score

105

Citations

2

Influential Citations

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Abstract

We present a membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from liquid-disordered phases coexisting in model membranes under both linear and nonlinear microscopies. The dye's fluorescence emission spectrum is blue-shifted 60 nm in liquid-ordered phases compared with liquid-disordered phases, and shows strong second harmonic generation in the liquid-disordered phase compared with the liquid-ordered phase. The ease of staining and the ability of this single dye to detect both phases, should lead to broad applications in biophysical studies of lipid domains in model membranes and cells.

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Di-4-ANEPPDHQ is a membrane-staining dye that differentiates liquid-ordered phases from liquid-disordered phases in model membranes, offering broad applications in biophysical studies of lipid domains in model membranes and cells.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.

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References

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

Di-propyl (di-3-ANEPPDHQ) and di-butyl (di-4-ANEPPDHQ) naphthylstyryl-pyridinium potentiometric dyes offer the best combination of signal-to-noise ratio, moderate phototoxicity,

Sensitivity of second harmonic generation from styryl dyes to transmembrane potential.

Second harmonic generation from styryl dyes can detect transmembrane potential with up to 43% sensitivity, depending on excitation wavelength and dye structure.

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Two-photon microscopy reveals that lipid rafts in living cells are primarily governed by cholesterol content, supporting the lipid raft hypothesis.

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Ternary mixtures of phospholipids and cholesterol can produce two coexisting liquid phases in giant vesicles, with interesting behavior like colliding, coalescing, and bulging out of the vesicle.

Lipid rafts reconstituted in model membranes.

In model membranes, lipid rafts can form, driven by lipid-lipid interactions, into more ordered, detergent-resistant domains, supporting the raft hypothesis.

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