Determination of naphthalene-2,3-dicarboxaldehyde-labeled amino acids by open tubular liquid chromatography with electrochemical detection.

doi:10.1021/AC00180A011

Paper

DOI: 10.1021/AC00180A011

Determination of naphthalene-2,3-dicarboxaldehyde-labeled amino acids by open tubular liquid chromatography with electrochemical detection.

Published Mar 1, 1989 · M. Oates, J. Jorgenson

Analytical chemistry

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Abstract

Naphthalene-2,3-dicarboxaldehyde (NDA) has been investigated as a new derivatizing reagent for the electrochemical detection of tagged amino acids. Gradient elution allowed for the separation of 18 NDA-derivatized amino acids on an open tubular liquid chromatography column in less than 50 min. Gradient elution and electrochemical detection were found to be compatible. A detection limit of 36 amol was obtained for the asparagine-NDA derivative. The usefulness of this technique for quantitation was demonstrated by the analysis of the NDA-tagged hydrolysis products from bovine chymotrypsinogen.

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Study Snapshot

Naphthalene-2,3-dicarboxaldehyde (NDA) is a promising derivatizing reagent for electrochemical detection of tagged amino acids, allowing for rapid separation and quantitation in less than 50 minutes.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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