Paper
Ecdysterone and Turkesterone in Ajuga turkestanica Determined by HPLC
Published Jul 1, 2005 · DOI · I. T. Abdukadirov, M. R. Yakubova, Kh. R. Nuriddinov
Chemistry of Natural Compounds
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Abstract
Shoots of Ajuga turkestanica (Rgl.) Brig. (Labiatae, Mint) are a source of highly effective biologically active compounds such as phytoecdysteroids and natural glycosides [1, 2]. Ecdysterone, cyasterone, ajugalactone, ajugasterone, α-ecdysone, and turkesterone have been isolated previously from A. turkestanica. Of these, the main components are ecdysterone and turkesterone, which have very high anabolic activities [1]. The ecdysteroid content, in particular, that of turkesterone, in the raw material was determined by gravimetry [3-5] or UV spectrophotometry [6]. The turkesterone content varied from 0.025 to 0.17, which means that A. turkestanica is not an industrial raw material. HPLC is known to determine with high accuracy the content of ecdysteroids in certain plant sources [7-9]. We investigated the contents of turkesterone and ecdysterone in the aerial part of A. turkestanica by HPLC on a Zorbax Eclipse XDB-C18 column, 3×150 mm, 3.5 μm (Agilent Technologies) using an Agilent LC 1100 chromatograph with a four-eluent pump, deaerator, and variable wavelength UV detector. The chromatograph was controlled and the results were processed using Agilent ChemStation software for the liquid chromatograph. UV detection of separate ecdysteroids was carried out at working wavelength 247 nm. The elution gradient is described below:
HPLC accurately determines the content of ecdysteroids and turkesterone in Ajuga turkestanica shoots, providing valuable information for understanding the plant's biological activity.
- PopulationOlder adults (50-71 years)
- Sample size24
- MethodsObservational
- OutcomesBody Mass Index projections
- ResultsSocial networks mitigate obesity in older groups.
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