An SH reactive agent, N-ethyl maleimide (10^−3 M for 2 min in the luminal fluid) selectively inhibits the urea transpithelial flux across the toad gall bladder. Thiourea and antipyrine flux is not inhibited. The inhibitory effect on urea flux seems to be exerted on the carrier mechanism of urea transport.

N-ethyl maleimide selectively inhibits urea transpithelial flux across the toad gall bladder, affecting the carrier mechanism of urea transport.

Paper

Effects of N-ethyl maleimide on urea facilitated transport across toad gall bladder

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