Exploited Application of a Newly Isolated Pseudomonas acidovorans XII in Microbial Degradation of 1-Chloro-4- Nitrobenzene

doi:10.12691/IJEBB-2-2-5

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Exploited Application of a Newly Isolated Pseudomonas acidovorans XII in Microbial Degradation of 1-Chloro-4- Nitrobenzene

Published Jan 23, 2014 · M. Shah

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Abstract

Bacterial strain XII, which belongs to the family Pseudomonad, utilizes 1-chloro-4-nitrobenzene as a sole source of carbon, nitrogen, and energy. Suspensions of 1-chloro-4-nitrobenzene -grown cells removed 1-chloro-4-nitrobenzene from culture fluids, and there was a concomitant release of ammonia and chloride. Under anaerobic conditions XII transformed 1-chloro-4-nitrobenzene into a product which was identified as 2-amino-5-chlorophenol by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. This transformation indicated that there was partial reduction of the nitro group to the hydroxylamino substituent, followed by Bamberger rearrangement. In the presence of oxygen but in the absence of NAD, fast transformation of 2-amino-5-chlorophenol into a transiently stable yellow product was observed with resting cells and cell extracts. This compound exhibited an absorption maximum at 395 nm and was further converted to a dead-end product with maxima at 226 and 272 nm. The compound formed was subsequently identified by 1H and 13C NMR spectroscopy and mass spectrometry as 5-chloropicolinic acid. In contrast, when NAD was added in the presence of oxygen, only minor amounts of 5-chloropicolinic acid were formed, and a new product, which exhibited an absorption maximum at 306 nm, accumulated.

Study Snapshot

Pseudomonas acidovorans XII effectively degrades 1-chloro-4-nitrobenzene into 2-amino-5-chlorophenol and 5-chloropicolinic acid under anaerobic conditions, with potential applications in bioremediation and bioremediation

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Exploited Application of a Newly Isolated Pseudomonas acidovorans XII in Microbial Degradation of 1-Chloro-4- Nitrobenzene

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References

Patchwork Assembly of nag-Like Nitroarene Dioxygenase Genes and the 3-Chlorocatechol Degradation Cluster for Evolution of the 2-Chloronitrobenzene Catabolism Pathway in Pseudomonas stutzeri ZWLR2-1

The 2-chloronitrobenzene catabolism pathway in Pseudomonas stutzeri ZWLR2-1 evolved by recruiting two catabolic clusters encoding a nitroarene dioxygenase and a chlorocatechol degradation pathway.

Nitroaromatic Compounds, from Synthesis to Biodegradation

Nitroaromatic compounds, a class of industrial chemicals, are harmful to human health and the environment, but bacteria in contaminated environments have evolved new biodegradation pathways to utilize them as carbon, nitrogen, and energy sources.

In situ bioremediation of monoaromatic pollutants in groundwater: a review.

In situ bioremediation effectively removes monoaromatic pollutants from groundwater, with enhanced anaerobic bioremediation being simple, applicable, and economical for BTEX contaminated groundwaters.

A Novel Deaminase Involved in Chloronitrobenzene and Nitrobenzene Degradation with Comamonas sp. Strain CNB-1

CnbZ is a novel enzyme in Comamonas strain CNB-1 that deaminates xenobiotic compounds and/or -amino acids, resulting in the release of ammonia for bacterial growth.

Two-year feed study of carcinogenicity and chronic toxicity of ortho-chloronitrobenzene in rats and mice.

Ortho-chloronitrobenzene (o-CNB) is a potent carcinogen and chronic toxicity agent in rats and mice, with higher dose levels meeting maximum tolerated dose criteria except for 2000 ppm in male rats.

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