Impact of linoleic acid on bovine oocyte maturation and embryo development.

doi:10.1530/REP-09-0503

Paper

Impact of linoleic acid on bovine oocyte maturation and embryo development.

Published Jun 1, 2010 · W. F. Marei, Claire D. Wathes, A. Fouladi-Nashta

Reproduction

Q1 SJR score

171

Citations

5

Influential Citations

Full textSemantic Scholar

Abstract

Linoleic acid (LA; 18:2 n-6) is the most abundant fatty acid in bovine follicular fluid, and it was previously reported that LA concentration significantly decreases when follicle size increases. This suggests that LA may have a role in the regulation of oocyte maturation. The present study investigated the effect of LA supplementation on bovine oocyte maturation and early embryo development in vitro. Treatment of cumulus-oocyte complexes (COCs) with LA significantly inhibited cumulus cell expansion and retarded development of the oocytes to the metaphase II (MII) stage in a dose-dependent manner. This effect was reversible, and the oocytes developed to the MII stage after extended culture in the absence of LA. Treatment of COCs with LA also resulted in a significantly lower percentage of cleaved embryos and blastocyst yield. Furthermore, COCs treated with LA had significant effects compared with controls in i) increasing prostaglandin E(2) concentration in the medium, ii) decreasing intracellular cAMP at 6 and 24 h of maturation and iii) decreasing phosphorylation of the MAPK1 and 3 at 24 h, and AKT at 6 h of maturation. In conclusion, LA supplementation to bovine oocytes during maturation altered the molecular mechanisms regulating oocyte maturation and resulted in decreased percentage of oocytes at MII stage and inhibition of the subsequent early embryo development. These data provide evidence for adverse effects of LA on oocyte development, which can be associated with dietary increased level of LA in the follicular fluid and the decline in fertility in farm animals and human.

In Vitro Study

Highly Cited

Study Snapshot

Linoleic acid supplementation in bovine oocytes during maturation can alter oocyte maturation mechanisms, leading to decreased oocyte development and inhibition of early embryo development.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Sign up to use Study Snapshot

Consensus is limited without an account. Create an account or sign in to get more searches and use the Study Snapshot.

Create an account

Paper

Impact of linoleic acid on bovine oocyte maturation and embryo development.

Sign up to use Study Snapshot

References

The Effect of Linolenic Acid on Bovine Oocyte Maturation and Development1

Linolenic acid supplementation in bovine oocyte maturation leads to increased metaphase II oocytes and improved early embryo development, mediated by the MAPK pathway and PGE2 synthesis.

Oocyte quality in lactating dairy cows fed on high levels of n-3 and n-6 fatty acids.

Oocyte quality in lactating dairy cows fed on high levels of n-3 and n-6 fatty acids shows only modest effects on their developmental potential due to the ovary buffering against fluctuations in plasma n-3 and n-6 fatty acids.

MAPK3/1 (ERK1/2) in Ovarian Granulosa Cells Are Essential for Female Fertility

ERK1/2 and C/EBP kinases in ovarian granulosa cells are essential for luteinizing hormone-induced ovulation and luteinization, which are crucial for female reproductive development.

Δ6‐Desaturase (FADS2) deficiency unveils the role of ω3‐ and ω6‐polyunsaturated fatty acids

FADS2 deficiency in mice leads to sterility and highlights the importance of 3 and 6polyunsaturated fatty acids for cell viability and membrane phospholipid function.

Effect of level of dietary n-3 polyunsaturated fatty acid supplementation on systemic and tissue fatty acid concentrations and on selected reproductive variables in cattle.

Increasing dietary n-3 polyunsaturated fatty acid supplementation in cattle increases uterine endometrial concentrations of EPA and total n-3 PUFA, but has no significant effect on plasma P4 or E2 concentrations.

Citations

The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine Oocytes

Adding PMSG and hCG to sheep oocyte maturation media improves oocyte maturation in vitro.

The vaginal metabolomics profile with features of polycystic ovary syndrome: a pilot investigation in China

Vaginal metabolomics can effectively differentiate between PCOS and healthy control groups, providing a more economical and harmless alternative for personalized health diagnosis and potential therapeutic targets.

Multi-Omics Reveals the Role of Arachidonic Acid Metabolism in the Gut–Follicle Axis for the Antral Follicular Development of Holstein Cows

Gut microbiota-related phosphocholine and linoleic acid play a crucial role in regulating follicular development and oocyte quality in subfertile dairy cows.

Genetic insights of blood lipid metabolites on polycystic ovary syndrome risk: a bidirectional two-sample Mendelian randomization study

Genetic evidence suggests a causal relationship between hexadecanedioate and dihomo-linolenate and the risk of PCOS, potentially serving as metabolic biomarkers for screening and selecting drug targets.

Extracellular Vesicles Contribute to the Difference in Lipid Composition between Ovarian Follicles of Different Size Revealed by Mass Spectrometry Imaging

Extracellular vesicles contribute to the lipid composition differences between dominant and subordinate follicles in ovarian follicles, potentially influencing oocyte competence and follicle growth.