Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

doi:10.3390/toxins8050139

Paper

Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

Published May 1, 2016 · Mingxing Feng, Zhenyu He, Yuanyuan Wang

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Abstract

Periplocosides, which are insecticidal compounds isolated from the root bark of Periploca sepium Bunge, can affect the digestive system of insects. However, the mechanism though which periplocosides induces a series of symptoms remains unknown. In this study, affinity chromatography was conducted by coupling periplocoside E-semi-succinic acid ester with epoxy amino hexyl (EAH) sepharose 4B. Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) was performed to analyze the fraction eluted by periplocoside E. Eight binding proteins (luciferin 4-monooxygenase, aminopeptidase N, aminopeptidase N3, nicotinamide adenine dinucleotide health (NADH) dehydrogenase subunit 5, phosphatidylinositol 3-phosphate 3-phosphatase myotubularin, actin, uncharacterized family 31 glucosidase KIAA1161, and 2OG-Fe(2) oxygenase superfamily protein) were obtained and identified through liquid chromatography/quadrupole-time of flight-mass spectrometry (LC/Q-TOF-MS) analysis of the midgut epithelium cells of Mythimna separata larvae. Aminopeptidase N and N3 are potential putative targets of periplocosides. This study establishes the foundation for further research on the mechanism of action and target localization of periplocosides in agricultural pests.

In Vitro Study

Study Snapshot

Periplocoside E from Periploca sepium Bunge root bark affects the digestive system of insects, with aminopeptidase N and N3 as potential targets for its insecticidal effects.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

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References

Citations

Genus Periploca (Apocynaceae): A Review of Its Classification, Phytochemistry, Biological Activities and Toxicology

The genus Periploca contains diverse natural products with potential medical and agricultural value, but further research is needed to ensure safety and avoid potential toxicities.

Antifungal activity of pregnane glycosides isolated from Periploca sepium root barks against various phytopathogenic fungi

Pregnane glycosides from Periploca sepium root barks show significant antifungal activity against various phytopathogenic fungi and are safe for human, mammals, and non-target organisms.

Comparative Proteomic Analysis of the Effect of Periplocoside P from Periploca sepium on Brush Border Membrane Vesicles in Midgut Epithelium of Mythimna separata Larvae

Periplocoside P from Periploca sepium may target the V-type ATPase A subunit in the midgut epithelium of Mythimna separata larvae, providing preliminary evidence for their mode of action against this insect.

Centipede venoms as a source of drug leads

Centipede venoms contain complex, disulfide-constrained peptides with novel pharmacological properties, offering potential for drug discovery and development.

Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography

Celangulin V binding proteins identified in the midgut of Mythimna separata larvae may serve as target proteins for its insecticidal activity.