An efficient process to synthesize 5′-O-dimethoxytrityl-N4-benzoyl-5-methyl-2 ′-deoxycytidine in high yield and quality is described. Final benzoylation was improved by developing a method to selectively hydrolyze benzoyl ester impurities. This inexpensive approach was scaled up to multi-kilogram quantities for routine use in oligonucleotide therapeutics.

This efficient process efficiently synthesizes 5′-O-dimethoxytrityl-N4-benzoyl-5-methyl-2′-deoxycytidine in high yield and quality for routine use in oligonucleotide therapeutics.

Paper

Efficient Large-Scale Synthesis of 5′-O-Dimethoxytrityl-N 4-Benzoyl-5-Methyl-2′-Deoxycytidine

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