Mechanism of the mutagenic action of hydroxylamine. VII. Functional activity and specificity of cytidine triphosphate modified with hydroxylamine and O-methylhydroxylamine.

doi:10.1016/0005-2787(72)90370-X

Paper

Mechanism of the mutagenic action of hydroxylamine. VII. Functional activity and specificity of cytidine triphosphate modified with hydroxylamine and O-methylhydroxylamine.

Published Dec 6, 1972 · E. I. Budowsky, E. D. Sverdlov, T. N. Spasokukotskaya

Biochimica et biophysica acta

UNKNOWN SJR score

30

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Abstract

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In Vitro Study

Study Snapshot

Hydroxylamine and O-methylhydroxylamine modify cytidine triphosphates, exhibiting a mutagenic action and amphoteric (dual) functional specificity in the RNA polymerase system.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Mechanism of the mutagenic action of hydroxylamine. VII. Functional activity and specificity of cytidine triphosphate modified with hydroxylamine and O-methylhydroxylamine.

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References

Mechanism of the mutagenic action of hydroxylamine. VI. Reaction of hydroxylamine with the uracil nucleus.

Hydroxylamine's mutagenic action involves the addition of neutral reagent molecules to the uracil nucleus, leading to isoxazolone and ribosylurea, or fission of the uracil nucleus, depending on pH and concentration of hydroxy

Methylated polydeoxyribocytidylic acid templates for RNA polymerase.

Methylated DNA templates can cause misincorporation of UMP and AMP, suggesting that methylation of cytosine in DNA may be a mutagenic event.

Tautomerism of nucleic bases and the template synthesis of polynucleotides.

Tautomerism of nucleic bases may contribute to spontaneous mutations of the transition type, and the ability of modified cytidine residues to act as uridine ones may also play a role in these transitions.

Mutagenic anologues of cytosine: RNA polymerase template and substrate studies.

Polynucleotide synthesis with ATP and UTP can replace GTP and UTP, with varying degrees of efficiency depending on the template used.

Mechanism of the mutagenic action of hydroxylamine. 3. Reaction of hydroxylamine and O-methylhydroxylamine with the cytosine nucleus.

The cytosine nucleus' reactivity towards hydroxylamine and O-methylhydroxylamine is influenced by reaction conditions, substitution at the cytosine nucleus, and the chemical properties of the modified products.

Citations

Selective chemical modification of DNA with alkoxy- and benzyloxyamines.

This study presents a new method for selective chemical modification of DNA at cytosine nucleobases using alkoxy- and benzyloxyamines, with benzyloxyamines being particularly effective.

Template properties of mutagenic cytosine analogues in reverse transcription

Cytidine analogues are highly mutagenic when incorporated into RNA, potentially making them potential antiretroviral agents by causing mutations in the viral genome.

Synthesis of N4-substituted CTP by mammalian CTP synthetase.

Mammalian CTP synthetase catalyzes the formation of N4-substituted CTP from UTP and hydroxylamine, with nucleophilicity and basicity of amines playing a crucial role in the enzyme's reaction with UTP.

Mutagenesis by N4-aminocytidine: induction of AT to GC transition and its molecular mechanism.

N4-aminocytidine induces mutagenesis in Escherichia coli and Salmonella typhimurium by inducing AT to GC transitions in DNA during cell multiplication, with its molecular mechanism being a cytosine analogue incorporated as a substitute for

Induction of mutation in vitro in phage phi X174 am3 by N4-aminodeoxycytidine triphosphate.

N4-aminodeoxycytidine triphosphate effectively induces mutation in phage phi X174 am3 in vitro, making it a potential reagent for in vitro site-directed mutagenesis.