Paraformaldehyde fixation in immunofluorescence and immunoelectron microscopy. Preservation of tissue and cell surface membrane antigens.

doi:10.1016/0022-1759(74)90093-3

Paper

DOI: 10.1016/0022-1759(74)90093-3

Paraformaldehyde fixation in immunofluorescence and immunoelectron microscopy. Preservation of tissue and cell surface membrane antigens.

Published Dec 1, 1974 · J. Smit, C. Meijer, F. Décary

Journal of immunological methods

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Abstract

Abstract hidden due to publisher request; this does not indicate any issues with the research. Click the full text link above to read the abstract and view the original source.

In Vitro Study

Study Snapshot

Fixation with 1% paraformaldehyde preserves tissue and membrane-bound antigens, while higher concentrations cause rapid loss of antigenicity and require immediate removal of cells for imaging.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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References

DOI: 10.1016/0022-1759(74)90056-8

Fixation of cell-bound antibody in the membrane immunofluorescence test.

PFA fixation of cell-bound antibody in membrane immunofluorescence tests effectively reduces redistribution phenomena and maintains the characteristic hand-mirror configuration of stimulated lymphocytes.

1974·49citations·Peter Biberfeld et al.·Journal of immunological methods

Journal of immunological methods

DOI: 10.1083/JCB.59.2.537

IMMOBILIZATION OF MEMBRANE H-2 ANTIGENS BY PARAFORMALDEHYDE FIXATION

Paraformaldehyde fixation immobilizes mouse H-2 antigens on the surface membrane, making them less susceptible to alterations caused by antibodies.

1973·35citations·E. Parr et al.·The Journal of Cell Biology

The Journal of Cell Biology

DOI: 10.1111/j.1365-3083.1972.tb03730.x

Surface‐Bound Immunoglobulin on Lymphocytes from Normal and Immunodeficient Humans

Surface-bound immunoglobulin is present on lymphocytes in normal humans, with IgM dominating the class and IgG2 the dominating subclass, and is absent in the blood of patients with severe hypogammaglobulinemia.

1972·145citations·S. FrØLand et al.·Scandinavian Journal of Immunology

Scandinavian Journal of Immunology

DOI: 10.1083/JCB.50.2.432

ULTRASTRUCTURAL LOCALIZATION OF INTRACELLULAR ANTIGEN USING ENZYME-LABELED ANTIBODY FRAGMENTS

Enzyme-labeled antibody fragments (Fab) are more effective for locating intracellular antigens in tissues than immunoglobulin G, with cytochrome c being a better choice for nonspecific labeling.

1971·48citations·J. Kraehenbuhl et al.·The Journal of Cell Biology

The Journal of Cell Biology

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THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE

The new ultrastructural cytochemical technique effectively studies early stages of protein absorption in mouse kidney proximal tubules, providing valuable insights for studying protein transport in various cells and tissues.

1966·6632citations·R. Graham et al.·Journal of Histochemistry and Cytochemistry

Journal of Histochemistry and Cytochemistry

DOI: 10.1016/0022-1759(73)90012-4

The use of peroxidase-labelled antiglobulin for ultrastructural localization of tissue antigens reacting with serum antibodies.

Peroxidase-conjugated antiglobulin can effectively localize tissue antigens reacting with serum antibodies, providing information not available through light microscopy.

1973·19citations·J. Webb et al.·Journal of immunological methods

Journal of immunological methods

Citations

DOI: 10.3390/cells10061302

The Impact of Fixation on the Detection of Oligodendrocyte Precursor Cell Morphology and Vascular Associations

Fixation methods impact the visibility of oligodendrocyte precursor cell morphology and interactions with the brain's vasculature, with stronger fixation affecting fluorescence intensity.

2021·10citations·Friederike Pfeiffer et al.·Cells

Cells

DOI: 10.1007/s10815-019-01666-9

PFA is superior to glyoxal in preserving oocyte, embryo, and stem cell proteins evidenced by super-resolution microscopical surveys of epitopes

PFA is superior to glyoxal in preserving cellular proteins in situ, especially in oocytes and embryos, for proper cell fixation.

2020·7citations·F. Çelikkan et al.·Journal of Assisted Reproduction and Genetics

Journal of Assisted Reproduction and Genetics

DOI: 10.1101/625863

Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of Epitopes

Glyoxal does not accurately preserve cellular proteins as accurately as paraformaldehyde for various cell types and proteins tested.

2019·2citations·F. Çelikkan et al.·bioRxiv

bioRxiv

DOI: 10.3390/membranes9020029

Antibacterial Properties of Polysulfone Membranes Blended with Arabic Gum

Polysulfone membranes blended with arabic gum show strong antibacterial properties, making them attractive for water treatment applications.

2019·32citations·Souhir Sabri et al.·Membranes

Membranes

DOI: 10.1039/c8lc00418h

A glass-based, continuously zonated and vascularized human liver acinus microphysiological system (vLAMPS) designed for experimental modeling of diseases and ADME/TOX.

The vLAMPS is a user-friendly, glass-based microfluidic device that allows for real-time imaging and investigation of human liver functions, including physiology, toxicology, and disease progression.

2018·86citations·Xiang Li et al.·Lab on a chip

Lab on a chip