PHOTOHEMOLYSIS OF HUMAN ERYTHROCYTES LABELED IN BAND 3 WITH EOSIN‐ISOTHIOCYANATE

doi:10.1111/j.1751-1097.1986.tb04698.x

Paper

PHOTOHEMOLYSIS OF HUMAN ERYTHROCYTES LABELED IN BAND 3 WITH EOSIN‐ISOTHIOCYANATE

Published Oct 1, 1986 · J. Pooler, A. Girotti

Photochemistry and Photobiology

23

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Abstract

Eosin‐isothiocyanate (EYNCS) is 50 to 100 times more effective in sensitizing delayed photo‐hemolysis of human erythrocytes than is eosin when matched for absorbance in the reaction medium. These dyes are equally efficient in generating singlet oxygen, a potent membrane oxidant. When cells are treated with sensitizer and washed extensively prior to illumination, EYNCS phototoxicity persists, while that of eosin is lost. SDS‐gel electrophoresis of membranes from EYNCS‐exposed cells shows a large fluorescence signal coincident with band 3 protein that is abolished by pretreatment with H2DIDS, the inhibitor of anion exchange by band 3. This treatment reduces the photohemolytic potency of EYNCS by over 90%. The marked enhancement of photohemolytic activity upon binding sensitizer to band 3 implicates band 3 itself as a site of photodamage leading to lysis.

Study Snapshot

Eosin-isothiocyanate (EYNCS) is a highly effective photo-hemolytic agent, causing photodamage to human erythrocytes, with band 3 protein being a key site of photodamage.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

PHOTOHEMOLYSIS OF HUMAN ERYTHROCYTES LABELED IN BAND 3 WITH EOSIN‐ISOTHIOCYANATE

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