p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.

p-Maleimidophenyl isocyanate is a novel heterobifunctional linker for thiol to hydroxyl coupling, useful for activating proteins and enzymes with thiol-containing structures.

Paper

p-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling.

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