Study of the toxin of Clostridium botulinum. Effects of 2-hydroxy-5-nitrobenzyl bromide on the biological activity of botulinum toxin.

doi:10.1016/0304-4165(66)90079-1

Paper

DOI: 10.1016/0304-4165(66)90079-1

Study of the toxin of Clostridium botulinum. Effects of 2-hydroxy-5-nitrobenzyl bromide on the biological activity of botulinum toxin.

Published Apr 25, 1966 · D. A. Boroff, B. Dasgupta

Biochimica et biophysica acta

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Abstract

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In Vitro Study

Study Snapshot

2-Hydroxy-5-nitrobenzyl bromide effectively reduced the toxicity of Clostridium botulinum Type A toxin by 99% and modified 17.8 moles of tryptophan, destroying its ability to stimulate protective antibidy formation in rabbits.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Effect of tetranitromethane on the biological activities of botulinum neurotoxin types A, B and E

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Reaction of 2-hydroxy-5-nitrobenzylated carboxypeptidase A with 14 C-1-fluoro-2,4-dinitrobenzene.

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Dimethyl (2-hydroxy-5-nitrobenzyl) sulfonium salts effectively introduce 2-hydroxy-5-nitrobenzyl reporter grouping into tryptophyl residues in proteins, with nearly quantitative yield and minimal by-product formation.

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Modification of carboxyl groups in botulinum neurotoxin types A and E.

Modification of three carboxyl groups in botulinum neurotoxin types A and E leads to over 95% detoxification, but complete detoxification cannot be achieved with these reagents.

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