Paper
Synthesis and metabolism of 5beta-[11,12-3H]cholestane-3alpha, 7alpha-diol.
Published Feb 1, 1978 ยท K. Okuda, Y. Atsuta
Journal of biochemistry
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Abstract
5beta-[11,12-3H]Cholestane-3alpha,7alpha-diol was synthesized as follows. 5beta-Cholestane-3alpha,7alpha,12atriol 3,7-diacetate was treated with phosphorus oxychloride in pyridine solution and then the product, 5beta-cholest-11-ene-3alpha,7alpha-diol diacetate, was hydrogenated in acetic acid solution using platinum oxide as a catalyst under an atmosphere of tritium gas. 5beta-[11,12-3H]Cholestane-3alpha,7alpha-diol thus obtained was readily hydroxylated at C-26 by mitochondria in the presence of isocitric acid, magnesium chloride and potassium cyanide.
5beta-[11,12-3H]cholestane-3alpha,7alpha-diol is a promising synthetic and metabolic tool for the study of cholestane metabolism in plants.
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