5-(4-Pyridyl)-2-thiophenecarbaldehyde has been prepared as a new fluorescent derivatization reagent and utilized for the determination of primary alkylamines. A definite detection limit was achieved for the amines by using a method based on the combination of precolumn derivatization and postcolumn hydrolysis–detection of the original fluorescent reagent regenerated from the derivatives. Approximately a 0.1 pmol (10 μl injection) (S/N=2) of detection limit was obtained. A dynamic linear range was over 5 orders of magnitude.

5-(4-Pyridyl)-2-thiophenecarbaldehyde is a new fluorescent reagent for detecting primary alkylamines with a 0.1 pmol detection limit and over 5 orders of magnitude dynamic linear range.

Paper

Thienylpyridines as a New Fluorescent Reagent. I. Determination of Primary Alkylamines with 5-(4-Pyridyl)-2-thiophenecarbaldehyde Using HPLC

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