Time course of inhibition of brain nitric oxide synthase by 7-nitro indazole.

doi:10.1097/00001756-199410000-00039

Paper

Time course of inhibition of brain nitric oxide synthase by 7-nitro indazole.

Published Oct 3, 1994 · G. Mackenzie, S. Rose, P. Bland-Ward

Neuroreport

Q3 SJR score

158

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2

Influential Citations

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Abstract

7-Nitro indazole (7-NI) inhibits rat striatal, cerebellar, hippocampal, cerebral cortex and olfactory bulb nitric oxide synthase (NOS) in vitro with IC50 values of 0.68 +/- 0.01 microM, 0.64 +/- 0.03 microM, 1.53 +/- 0.05 microM, 0.93 +/- 0.04 microM and 1.05 +/- 0.02 microM respectively (n = 6). Intraperitoneal (i.p.) or oral administration of 7-NI (30 mg kg-1) to rats inhibited NOS enzyme activity measured ex vivo in all five brain regions (n = 5-6). NOS inhibition (maximal effect, 0.5 h post-injection) was transient with complete recovery at either 4 h (oral administration) or 24 h (i.p. administration). Repeated i.p. injection of 7-NI (30 mg kg-1, every 4 h for 20 h) inhibited NOS enzyme activity at 24 h by 51-61% in all brain regions. The relatively transient NOS inhibitory effect of 7-NI following parenteral administration should be taken into account when using this drug to evaluate the central effects of nitric oxide.

Non-RCT

Animal Study

Highly Cited

Study Snapshot

7-Nitro indazole effectively inhibits brain nitric oxide synthase, but its effect is relatively transient, requiring careful consideration when evaluating central effects of nitric oxide.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Paper

Time course of inhibition of brain nitric oxide synthase by 7-nitro indazole.

References

Citations

Activation of NOS-cGMP pathways promotes stress-induced sensitization of behavioral responses in zebrafish

Different NO-related pathways converge at different time windows to induce stress-induced sensitization in adult zebrafish, with different inhibitory effects on stress-induced behavior.

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The single-dose PSARA gel formulation effectively improves memory, social interaction, and reduces anxiety-like behavior in Shank3 mutant mice, potentially offering a rational design for an effective ASD treatment.

Neuronal Nitric Oxide Inhibitor 7-Nitroindazole Improved Brain-Derived Neurotrophic Factor and Attenuated Brain Tissues Oxidative Damage and Learning and Memory Impairments of Hypothyroid Juvenile Rats

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Chronic continuous hypoxia reduces infarct size and risk ratio in rats by activating PKC, iNOS, and mitoKATP channels, while nNOS, PI3 kinase, sarcKATP channel, and MPT pore are not involved.

The role of neuronal nitric oxide synthase in cocaine place preference and mu opioid receptor expression in the nucleus accumbens

Neuronal nitric oxide synthase (nNOS) plays a role in regulating mu opioid receptor expression after acute cocaine administration, potentially identifying useful therapeutic targets for cocaine addiction.