pmi estimation of human skeletal remains using different spectroscopy methods

Introduction

Estimating the post-mortem interval (PMI) of human skeletal remains is a critical task in forensic science, aiding in the identification of the deceased and the determination of the time since death. Various spectroscopy methods have been explored to develop reliable and accurate techniques for PMI estimation.

Key Insights

Infrared (IR) Spectroscopy and Raman Spectroscopy:
- Both IR spectroscopy and Raman microscopy show potential for forming the basis of a quantitative method for PMI estimation .
Fourier Transform Infrared (FTIR) Spectroscopy:
- FTIR spectroscopy, combined with chemometrics, can effectively estimate PMI by analyzing changes in bone chemistry. The method shows promise with satisfactory prediction errors for both buried and unburied bones.
Radiometric Methods:
- The use of radiometric methods involving (90)Sr and (210)Pb can yield reliable PMI estimates, provided there is a calibration curve for the studied area. Adding (210)Po activity to (90)Sr dating increases reliability.
Citrate Content Analysis:
- Citrate content in bones decreases with increasing PMI, making it a promising method for PMI estimation. This method is easy and inexpensive to apply, though it requires further research for validation.
Combination of Methods:
- Combining C-14 analysis with luminol tests and histological examination can provide preliminary PMI indications. While C-14 is the most reliable, it is expensive. Luminol and histological methods are less reliable but useful for quick, preliminary assessments.

Conclusion

Various spectroscopy methods, including IR, Raman, and FTIR spectroscopy, show significant potential for estimating the PMI of human skeletal remains. Radiometric methods and citrate content analysis also offer promising approaches. Combining different methods, such as C-14 analysis with luminol tests and histological examination, can enhance the reliability and speed of PMI estimation. Further research and validation are needed to refine these techniques for practical forensic applications.

Pmi estimation of human skeletal remains using different spectroscopy methods

Summary

Introduction

Key Insights

Conclusion

Sources and full results

Estimating the Post-Mortem Interval of skeletonized remains: The use of Infrared spectroscopy and Raman spectro-microscopy

Infrared spectroscopy and Raman microscopy show potential for a quantitative method to estimate the Post-Mortem Interval (PMI) of skeletonized remains, aiding in identification and cause of death investigations.

Dating human skeletal remains using a radiometric method: biogenic versus diagenetic 90Sr and 210Pb in vertebrae.

Using (90)Sr and (210)Po radiometric methods to determine the post-mortem interval of human skeletal remains can provide reliable estimates up to 50 years after death.

A test of the citrate method of PMI estimation from skeletal remains.

Citrate content in bone decreases with increasing postmortem interval, making it an easy and inexpensive method for estimating PMI from skeletal remains.

Estimation of the late postmortem interval using FTIR spectroscopy and chemometrics in human skeletal remains.

FTIR spectroscopy and chemometrics can effectively estimate the postmortem interval of human remains, with potential for real-life forensic applications in natural conditions.

The comparative performance of PMI estimation in skeletal remains by three methods (C-14, luminol test and OHI): analysis of 20 cases

Combining luminol test and microscopic analysis may provide a promising solution for estimating post-mortem interval in forensic anthropology, with only two false-positive results and no false negatives.

Application of Micro-Computed Tomography for the Estimation of the Post-Mortem Interval of Human Skeletal Remains

Micro-computed tomography is an objective and precise method for estimating the post-mortem interval of human skeletal remains, with a deep learning approach showing high precision.

Assessing various Infrared (IR) microscopic imaging techniques for post-mortem interval evaluation of human skeletal remains

Infrared microscopic imaging techniques, such as reflection-, ATR-, and Raman-, can effectively estimate the post-mortem interval of human skeletal remains.

Preservation state assessment and post-mortem interval estimation of human skeletal remains using ATR-FTIR spectra

ATR-FTIR spectroscopy effectively assesses preservation state and estimates post-mortem intervals of human skeletal remains, considering skeletal element and environmental conditions.

Postmortem interval of skeletal remains through the detection of intraosseal hemin traces. A comparison of UV-fluorescence, luminol, Hexagon-OBTI®, and Combur® tests.

Lack of luminescence and reduced UV-fluorescence are more meaningful for estimating postmortem interval in skeletal remains than a positive luminol reaction or strong UV-fluorescence, while the Combur® test strips and Hexagon-OBTI® test are not suitable for this purpose.

Collagen degradation as a possibility to determine the post-mortem interval (PMI) of animal bones: a validation study referring to an original study of Boaks et al. (2014)

The Sirius Red/Fast Green Collagen Staining Kit effectively estimates the post-mortem interval in animal bones, with a correlation between PMI and collagen degradation.

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