What can crispr be used for
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Applications of CRISPR Technology
CRISPR in Genome Engineering
CRISPR-Cas9 technology has revolutionized genome engineering by enabling precise, site-specific DNA cleavage in both single cells and whole organisms. This has facilitated various applications in research, such as controlling transcription, modifying epigenomes, conducting genome-wide screens, and imaging chromosomes1. The technology's versatility extends to therapeutic applications, including the treatment of genetic disorders in animals and potential clinical use for human diseases of the eye and blood1.
CRISPR in Infectious Disease Diagnosis and Therapy
CRISPR technology is transforming the diagnosis and treatment of infectious diseases. It allows for the rapid, low-cost detection of pathogens and identification of drug-resistance genes2. Therapeutic strategies using CRISPR include targeting pathogenic DNA or RNA sequences, cleaving integrated viral genomes, and reprogramming human B cells to produce neutralizing antibodies2. CRISPR-based therapies are being developed for emerging viruses like SARS-CoV-2, with ongoing efforts to mitigate risks such as off-target effects2.
CRISPR in Cancer Research and Therapeutics
CRISPR has significantly advanced cancer research by enabling the development of study models and genetic screening studies to discover and validate therapeutic targets5. It is also being adapted to improve immunotherapies, enhance their potency, reduce toxicity, and lower manufacturing costs5. Additionally, CRISPR can be used for cancer detection and the development of anticancer therapies, including the inhibition of tumor growth5.
CRISPR in Agricultural Improvement
In agriculture, CRISPR technology is used to improve crop yield, biofortification, and tolerance to biotic and abiotic stresses4. It has been applied to various crops, with rice being the most studied4. CRISPR enables precise targeting of biological pathways, allowing for the modification of proteins, starch, oil, and other functional components, thereby enhancing crop performance and resilience3 4.
CRISPR in Industrial Biotechnology
CRISPR-Cas systems are utilized in industrial biotechnology for microbial engineering applications, including bacterial strain typing, immunization of cultures, and metabolic pathway engineering for improved biochemical synthesis6 7. These applications are crucial for the production of high-value metabolites, biofuels, and pharmaceutical products6.
CRISPR in Stem Cell Engineering and Regenerative Medicine
CRISPR technology is pivotal in stem cell engineering and regenerative medicine. It is used for in vitro manipulation of stem cell fate and in vivo applications such as preventing retinal and muscular degeneration, neural regeneration, and bone regeneration8. CRISPR also aids in the treatment of diseases affecting blood, skin, and liver, highlighting its potential in regenerative therapies8.
CRISPR in Human Disease Treatment
CRISPR-Cas9 has been applied to correct disease-causing DNA mutations in various genetic disorders, including Barth syndrome, Duchenne muscular dystrophy, hemophilia, β-Thalassemia, and cystic fibrosis9. It is also used in immunology-focused applications, such as targeting specific genes for AIDS treatment and promoting anti-tumor immunotherapy9. Additionally, CRISPR is combined with induced pluripotent stem cells for tissue engineering and disease modeling9.
CRISPR in Next-Generation Diagnostics
CRISPR technology promises rapid, accurate, and portable diagnostic tools for infectious diseases. It enables the detection of pathogens like Zika virus, Dengue virus, and human papillomavirus, as well as gene mutations in cancer patients10. These diagnostic tools are essential for early case detection, disease control, and timely clinical care, especially in remote settings10.
Conclusion
CRISPR technology has a wide range of applications across various fields, including genome engineering, infectious disease diagnosis and therapy, cancer research, agricultural improvement, industrial biotechnology, stem cell engineering, and human disease treatment. Its versatility and precision make it a powerful tool for advancing scientific research and developing innovative therapeutic and diagnostic solutions.
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Most relevant research papers on this topic
Applications of CRISPR technologies in research and beyond
CRISPR technologies enable efficient, site-specific genome engineering in single cells and whole organisms, with potential applications in research, medicine, agriculture, and controlling disease-carrying insects.
Rigorous Journal
Highly CitedCRISPR-based strategies in infectious disease diagnosis and therapy
CRISPR systems have broad applicability in infectious diseases, offering solutions to many of the most challenging human infections through rapid, low-cost diagnostic systems and targeted therapeutic approaches.
Systematic ReviewCRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement
CRISPR/Cas technology revolutionizes biomedical and agricultural fields by enhancing disease detection, gene therapy, and crop improvement.
Highly CitedUse of CRISPR systems in plant genome editing: toward new opportunities in agriculture
CRISPR systems in plants can improve yield performance, biofortification, and stress tolerance, with rice being the most studied crop.
Systematic ReviewHighly CitedCRISPR–Cas: a tool for cancer research and therapeutics
CRISPR systems have revolutionized cancer research by accelerating the development of study models, facilitating the discovery of new targets, and potentially aiding in cancer detection and therapy development.
Highly CitedApplications of CRISPR/Cas System to Bacterial Metabolic Engineering
CRISPR/Cas technology can increase bacterial productivity and yield by controlling metabolic flux, with potential applications in biochemical, biofuel, and pharmaceutical production.
Highly CitedAdvances in Industrial Biotechnology Using CRISPR-Cas Systems.
CRISPR-Cas systems offer efficient and easily programmable tools for precision breeding, microbial engineering, and biochemical synthesis in industrial biotechnology applications.
Highly CitedCRISPR technologies for stem cell engineering and regenerative medicine.
CRISPR technologies show promise in stem cell engineering and regenerative medicine, offering potential for treating diseases like retinal and muscular degeneration, neural regeneration, bone regeneration, and cartilage tissue engineering.
CRISPR-mediated genome editing and human diseases
CRISPR/Cas9 technology has shown promise in treating human diseases, enabling the creation of disease models and tissue engineering.
Highly CitedNext-generation diagnostics with CRISPR
CRISPR-Cas biology offers rapid, accurate, and portable diagnostic tools for infectious and noninfectious diseases, revolutionizing molecular diagnostics for infectious and noninfectious diseases.
Highly Cited