Wang Ke-xia
2007
Citations
0
Influential Citations
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Citations
Quality indicators
Journal
Chinese Journal of Experimental Traditional Medical Formulae
Abstract
Objective:To study the in vitro inhibitory of protoporphyrin disodium(PPN) on Hepatitis C virus.Methods:The different concentration of PPN and ribavirin water solution were respectively added into HCV Replicon cell medium.The cell toxicity of PPN was determined with MTT method.Real-time PCR was used for determining secrected HCV RNA in the culture medium.Results:PPN and ribavirin had no obvious cell toxicity at the concentration of 1 mg/mL.When the drug concentration of PPN was 1 mg/mL,inhibitive rates of HCV RNA was 73.44%.The experimental group and control group had striking difference(P0.05),When the drug concentration of ribavirin was 1 mg/mL,inhibitive rates of HCV RNA was 40.78%.EC_(50) were 0.23 mg·mL(-1) and 1.19 mg·mL(-1) respectively.Conclusion:PPN can inhibit duplication of Hepatitis C Virus in Vitro and its effect was better than ribavirin.