L. Ignarro, B. Barry, D. Gruetter
May 14, 1980
Citations
1
Influential Citations
94
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Summary The S-nitroso derivatives of cysteine, penicillamine, 2-mercaptoethylamine, glutathione, dithiothreitol, β-D-thioglucose and other thiols markedly activated unpurified and partially purified hepatic soluble guanylate cyclase. Heme was not required for enzyme activation since partially purified enzyme preparations were devoid of detectable heme. Guanylate cyclase activation by S-nitrosothiols was inhibited by hemoproteins and electrophilic agents. The requirement of thiols for activation of partially purified guanylate cyclase, and the marked enhancement by thiols of activation of unpurified guanylate cyclase, by nitroprusside and nitrosoguanidine suggest that the latter compounds reacted with thiols to form S-nitrosothiols which were potent activators of guanylate cyclase.