Guo-liang Li, Guang Chen, Yueting Liu
May 1, 2012
Citations
1
Influential Citations
12
Citations
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Abstract
A sensitive and selective method using 2-(7H-dibenzo[a,g]carbazol-7-yl)ethyl 4-methylbenzenesulfonate (DBCETS) as a new fluorescent labeling reagent has been proposed for simultaneously detecting BA and FFA by HPLC with fluorescence detector. The developed method offered the low detection limits of 0.42-0.70 and 0.28-0.57 ng/mL for BA and FFA, respectively. Compared with the reported methods, the proposed method here is capable of offering higher detection sensitivity and selectivity, with less cost and lower volume of sample preparation. This method was validated to ensure high accuracy and precision, and the reliability of its results. When applied to the serum samples of healthy volunteers and patients with hepatic carcinoma, it showed excellent applicability.