G. Ellman
Apr 1, 1958
Citations
33
Influential Citations
981
Citations
Quality indicators
Journal
Archives of biochemistry and biophysics
Abstract
Abstract 1. 1. A method for the determination of mercaptans is described. It depends on the interchange of bis( p -nitrophenyl)disulfide and mercaptan anions at pH 8.0. 2. 2. A study of the specificity of the reaction indicates that it occurs only with the S − group (actual or potential, as in thiourea). 3. 3. Heavy-metal ions interfere with the reaction by irreversibly bonding any S − ions. 4. 4. The kinetics of the reaction with cysteine and β-mercaptoethylamine were studied. 5. 5. Several applications of this reaction were made: ( a ) the determination of mercaptan in synthetic compounds; ( b ) mercaptan reactivity in proteins; and ( c ) determination of blood and serum concentrations.