G. M. Richards
Feb 1, 1974
Citations
4
Influential Citations
612
Citations
Quality indicators
Journal
Analytical biochemistry
Abstract
A modification of the diphenylamine procedure of Giles and Myers (2) is described which has 30% higher sensitivity and a lower reagent blank than the parent procedure, and is more conveniently carried out. Acetaldehyde in the procedure is replaced by paraldehyde, which is easier to store and which, unlike acetaldehyde, can be included in the diphenylamine reagent with little or no loss of reagent stability. The reagent is useful for about 1 month after preparation. The extraction method of Abraham et al. (5) for further increasing sensitivity is applicable to this modified procedure.