C. Qiu, Wang Ying-zhen
2005
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Journal
Analytical Laboratory
Abstract
In the britton-robison medium with pH 2.0,light green SF(yellowish) can bind with protein at room temperature to form a complex,which has a maximum absorption wavelength at 665 nm.The optimum reaction conditions,binding constant and maximum binding number of light green SF to proteins were investigated by spectrophotometry,and a new method for the determination of proteins was developed based on this binding reaction.The linear range for the determination of bovine serum albumin(BSA),human serum albumin(HAS),and Human Immunoglobulin G(IgG) can reach more than 80 μg/mL with the apparent molar absorptivity of 7.25×10~5,7.22×10~5 and 1.84×10~6 L·mol~(-1)·cm~(-1),respectively.A lot of foreign substances do not interfere with the determination of proteins except for the anionic surfactants and cationic surfactants.The results of the determination for the total proteins in human serum samples obtained by this new proposed method,which is of good selectivity and high sensitivity,agree well with those obtained by the coomassie brillant blue G-250 method.