14-Deoxycoleon U-induced endoplasmic reticulum stress-mediated apoptosis, autophagy, and cell cycle arrest in lung adenocarcinoma

doi:10.2147/OTT.S211933

Paper

14-Deoxycoleon U-induced endoplasmic reticulum stress-mediated apoptosis, autophagy, and cell cycle arrest in lung adenocarcinoma

Published Jul 30, 2019 · Xiaozhi Peng, Yi-jun Tu, S. Fu

OncoTargets and therapy

Q2 SJR score

4

Citations

0

Influential Citations

Full textSemantic Scholar

Abstract

Objective 14-Deoxycoleon U is a natural abietane-type diterpene and exerts an inhibitory effect on tumor cells proliferation, which suggests that 14-Deoxycoleon U may be a potent anti-cancerous lead compound for lung cancer treatment. This study was to evaluate potential of 14-Deoxycoleon U to treat lung adenocarcinoma in vitro and in vivo. Methods In the present study, the cell viability and apoptosis morphology of 14-Deoxycoleon U-treated A549 and LLC cells were explored using cell counting kit-8 assay and Hoechst 33258 staining. Then, the protein expressions about apoptosis, endoplasmic reticulum (ER) stress, autophagy and cell cycle were measured using Western blot. The autophagosome formation of 14-Deoxycoleon U-treated A549 cells was visualized using a confocal microscopy. LLC lung adenocarcinoma model was established. Results The results indicated that 14-Deoxycoleon U significantly inhibited A549 and LLC cell proliferation in a dose-dependent manner via caspase-dependent apoptosis. Furthermore, apoptosis of both cells was mediated by 14-Deoxycoleon U-induced ER stress. In addition, 14-Deoxycoleon U-induced A549 and LLC cell autophagy, thus promoting their death. Moreover, 14-Deoxycoleon U-induced cell cycle arrest in both cells via inhibition of cyclin D3, cyclin-dependent kinase 6, CDC2 and up-regulation of p21. In vivo results showed that administration of 14-Deoxycoleon U significantly suppressed LLC growth and adverse effects of 14-Deoxycoleon U on organs might be lower than of adriamycin. Conclusion Overall, our results demonstrated that 14-Deoxycoleon U represses in vitro and in vivo growth of lung adenocarcinoma through ER stress-mediated apoptosis accompanied by autophagy and cell cycle arrest.

In Vitro Study

Rigorous Journal

Study Snapshot

14-Deoxycoleon U effectively suppresses lung adenocarcinoma growth in vitro and in vivo through ER stress-mediated apoptosis, autophagy, and cell cycle arrest.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Sign up to use Study Snapshot

Consensus is limited without an account. Create an account or sign in to get more searches and use the Study Snapshot.

Create an account

Paper

14-Deoxycoleon U-induced endoplasmic reticulum stress-mediated apoptosis, autophagy, and cell cycle arrest in lung adenocarcinoma

Sign up to use Study Snapshot

References

Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

Coleus forskohlii extract significantly alleviates asthma symptoms by modulating inflammation and the extracellular matrix, potentially benefiting patients with asthma.

Resveratrol protects photoreceptors by blocking caspase‐ and PARP‐dependent cell death pathways

Resveratrol protects retinal photoreceptor cells by blocking caspase- and PARP-dependent cell death pathways, potentially restoring vision in retinal degeneration.

A novel anti‐cancer role of &bgr;‐apopicropodophyllin against non‐small cell lung cancer cells

-apopicropodophyllin (APP) shows strong anti-cancer activity against non-small cell lung cancer cells by inducing apoptosis through microtubule disruption, DNA damage, cell cycle arrest, and ER stress.

Pinocembrin induces ER stress mediated apoptosis and suppresses autophagy in melanoma cells.

Pinocembrin induces ER stress-mediated apoptosis and suppresses autophagy in melanoma cells, suggesting potential for melanoma therapy.

Current WHO guidelines and the critical role of immunohistochemical markers in the subclassification of non-small cell lung carcinoma (NSCLC): Moving from targeted therapy to immunotherapy.

Current WHO guidelines emphasize the importance of subclassifying non-small cell lung carcinoma (NSCLC) and the utility of immunohistochemical markers in accurate diagnosis and patient care.

Citations

Potential Therapeutic Action of Autophagy in Gastric Cancer Managements: Novel Treatment Strategies and Pharmacological Interventions

Targeting autophagy with small molecule activators or inhibitors may improve gastric cancer treatment and prevention by overcoming drug resistance.

Facing Cell Autophagy in Gastric Cancer – What Do We Know so Far?

Autophagy plays a crucial role in gastric cancer development and its targeted application in cancer treatment shows promise.

The Therapeutic Effect of Melatonin on GC by Inducing Cell Apoptosis and Autophagy Induced by Endoplasmic Reticulum Stress

Melatonin inhibits gastric cancer cell growth and invasion by inducing cell apoptosis and autophagy in response to endoplasmic reticulum stress.

The Therapeutic Effect of Melatonin on GC by Inducing Cell Apoptosis and Autophagy Induced by Endoplasmic Reticulum Stress

Melatonin inhibits gastric cancer cell growth and invasion by inducing cell apoptosis and autophagy in response to endoplasmic reticulum stress.