A. Sanghvi, C. Wight, B. Parikh
Nov 1, 1973
Citations
0
Influential Citations
22
Citations
Journal
American journal of clinical pathology
Abstract
A new reagent system with p-hydrazinobenzenesulfonic acid in phosphoric acid for the determination of urinary 17-hydroxycorticosteroids is described. Use of this reagent results in increased sensitivity of detection of compounds possessing a dihydroxyacetone side chain while it retains the same specificity as obtained with the Porter-Silber reagent. The reaction conditions employed with the new reagent allow the assay to be performed within 4 to 8 hours after hydrolysis of the samples of urine.