M. McNamara, J. Schmitt, R. Wykle
Jul 31, 1984
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Journal
Biochemical and Biophysical Research Communications
Abstract
Summary The human promyelocytic leukemia cell line HL-60 can be differentiated to cells resembling either neutrophils or mononuclear phagocytes by a diverse group of stimuli. However, the underlying mechanisms remain unknown. We report that 1- O -hexadecyl-2-acetyl- sn -glycerol inhibits the growth of HL-60 cells and induces differentiation to cells resembling mononuclear phagocytes. HL-60 cultures incubated for 6 days with 1- O -hexadecyl-2-acetyl- sn -glycerol (5 μg/ml) demonstrated a ten-fold increase in nonspecific esterase activity, and produced cells with morphological features similar to those of monocytes and macrophages. Higher concentrations of 1- O -hexadecyl-2-acetyl- sn -glycerol significantly inhibited the growth of HL-60 cells and resulted in the virtual absence of cells resembling the original HL-60 line. 1- O -Oleoyl-2-acetyl- rac -glycerol added under the same conditions did not induce cell differentiation or inhibit cell growth.