J. Wilkinson, F. Jenkins, G. Tuey
Mar 1, 1968
Citations
0
Influential Citations
4
Citations
Journal
Clinica chimica acta; international journal of clinical chemistry
Abstract
Abstract Prolonged storage of 2-oxobutyric acid for periods exceeding two years leads to the formation of the cyclic decomposition products, 2-ethyl-3-methyl-tetrahydrofuran-4, 5-dione-2-carboxylic acid and 2-ethyl-3-methyltetrahydrofuran-4,5-dione. The cyclic carboxylic acid has no demonstrable effect upon the activity of crystalline ox-heart lactate dehydrogenase or the human serum enzyme, but the dihydrofuranone strongly inhibits the enzyme from both sources. The inhibition appears to be of the “uncompetitive” type. Sodium 2-oxobutyrate appears to be more stable than the free acid and is therefore recommended for use as a substrate for the “2-hydroxybutyrate dehydrogenase” (HBD) reaction.