Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.

doi:10.1093/CLINCHEM/26.2.227

Paper

Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.

Published Feb 1, 1980 · P. Fossati, L. Prencipe, G. Berti

Clinical chemistry

Q1 SJR score

1,200

Citations

21

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Abstract

A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus. This chromogen system has a high absorptivity, affording useful results with sample/reagent volume ratios as low as 0.025. The procedure is applicable to serum, plasma, or diluted urine. A single working reagent is used; the reaction is complete in less than 15 min at room temperature. The red dye formed is measured at 520 nm; a blank sample measurement is not needed. The standard curve for the method is linear for uric acid concentrations up to 1500 mumol/L. Average analytical recovery of uric acid in human sera and urine exceeded 99%; within-run and between-run precision studies showed CV's of less than or equal to 1.2 and less than or equal to 2.2%, respectively. The new procedure correlated well with the uricase/catalase and uricase/ultraviolet methods. The method is suitable for automation.

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Study Snapshot

The new uric acid assay method using a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system provides accurate results in less than 15 minutes, with high absorptivity and potential for automation.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.

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References

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Bilirubin interferes with hydrogen peroxide detection in reagent systems due to chemical and spectral effects, affecting results.

Determination of uric acid on continuous-flow (AutoAnalyzer II and SMA) systems with a uricase/phenol/4-aminophenazone color test.

This new colorimetric method for uric acid assay on continuous-flow systems offers a significantly improved routine test for clinical laboratories compared to the commonly used phosphotungstate method.

Evidence about the catecholoxidase activity of the enzyme ascorbate oxidase extracted from Cucurbita pepo medullosa.

Ascorbate oxidase from Cucurbita pepo medullosa exhibits secondary catecholoxidase activity at pH 6.7, potentially impacting the darkening process of fruits and vegetables.

Studies on horseradish peroxidase. X. The mechanism of the oxidation of p-cresol, ferrocyanide, and iodide by compound II.

Horseradish peroxidase's mechanism involves intramolecular general acid catalysis, with specific acid-catalyzed mechanisms occurring in sufficiently acidic solutions.

A direct colorimetric determination of uric acid in serum and urine with uricase-catalase system.

This new method for colorimetric determination of uric acid in serum and urine using uricase and catalase shows good reproducibility and accuracy, making it suitable for routine use in clinical laboratories.

Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor

This study developed a two-solution technique for determining glucose in blood using glucose oxidase and 4-amino phenazone, resulting in a stable color development in 10 minutes and a high stability reagent for manual and automated methods.

Measurement of Serum Uric Acid in Great Britain in 1963

Serum uric acid measurements in gout studies should be subjected to rigorous peer review and cross-checking to ensure accurate results and avoid misdiagnosis.

Spectrophotometric Assay of Ascorbic Acid Oxidase

This method is based on the principle that the rate of oxygen consumption during ascorbic acid oxidation is proportional to the amount of enzyme present, making it a reliable and accurate method for measuring ascorbic acid.

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