M. E. Kauffman, W. Keener, S. Clingenpeel
Dec 1, 2003
Citations
1
Influential Citations
14
Citations
Journal
Journal of microbiological methods
Abstract
3-hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.