7beta-hydroxycholesterol induces apoptosis and regulates cyclooxygenase 2 in head and neck squamous cell carcinoma.

doi:10.1001/archoto.2008.558

Paper

7beta-hydroxycholesterol induces apoptosis and regulates cyclooxygenase 2 in head and neck squamous cell carcinoma.

Published Mar 1, 2009 · G. Heiduschka, B. Erovic, L. Vormittag

Archives of otolaryngology--head & neck surgery

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Abstract

OBJECTIVE To determine whether treatment with 7beta-hydroxycholesterol (7beta-HC) would trigger cell death in head and neck squamous cell carcinoma (HNSCC) cell lines in a dose-dependent fashion. DESIGN In vitro study. SUBJECTS The study included HNSCC cell lines SCC9, SCC25, CAL27, and FaDu. INTERVENTION We treated HNSCC cell lines with increasing doses of 7beta-HC. Proliferation assays were performed to assess cell viability after treatment. Western blots were carried out to evaluate cyclooxygenase (COX)-1 and -2 expression levels. RESULTS Using proliferation assays and immunocytochemical analysis, we detected significant growth inhibition via apoptosis in 4 different HNSCC cell lines after treatment with 7beta-HC (P < .001). The 50% inhibitory concentration levels were between 13.19 and 20.79 micromol/L after 72 hours. Western analysis indicated that COX-2, but not COX-1, levels were suppressed after treatment. CONCLUSIONS Treatment with 7beta-HC resulted in suppression of HNSCC growth in vitro. Our data warrant further investigations for the potential use of 7beta-HC as a cytotoxic agent in head and neck cancer.

In Vitro Study

Study Snapshot

7beta-hydroxycholesterol treatment effectively suppresses head and neck squamous cell carcinoma growth in vitro, warranting further investigation as a potential cytotoxic agent in head and neck cancer.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

7beta-hydroxycholesterol induces apoptosis and regulates cyclooxygenase 2 in head and neck squamous cell carcinoma.

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References

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Gefitinib 250 mg daily shows less activity than 500 mg daily in recurrent and metastatic head and neck cancer, with cutaneous toxicity grade being a clinical predictor of better outcome.

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Cancer death rates have declined while guideline-based treatment has increased, but progress is not equally shared across all racial and ethnic populations.

The role of the mitochondria in apoptosis induced by 7β-hydroxycholesterol and cholesterol-5β,6β-epoxide

7-hydroxycholesterol-induced apoptosis occurs via the mitochondrial pathway, while cholesterol-5,6-epoxide induces caspase-8 activity but has no effect on mitochondrial membrane potential or cytochrome c release in U937 cells.

Citations

Impact of Oxysterols on Cell Death, Proliferation, and Differentiation Induction: Current Status

Oxysterols can stimulate osteogenic differentiation of mesenchymal stem cells, potentially benefiting cancer treatment and regenerative medicine.

Evaluation of Cytotoxicity Effects of Chalcone Epoxide Analogues as a Selective COX-II Inhibitor in the Human Liver Carcinoma Cell Line

Chalcone epoxide analogues show significant growth-inhibitory effects on the HepG2 cell line, suggesting potential for efficient COX-2 dependent cancer chemotherapy.

Effect of the histone deacetylase inhibitor resminostat on head and neck squamous cell carcinoma cell lines

Resminostat, a histone deacetylase inhibitor, shows potential in treating head and neck squamous cell carcinoma by targeting epigenetic events.

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Neratinib, as a single agent or in combination with chemo-irradiation, shows potential as a promising treatment option for patients with head and neck cancer.

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PLK1 inhibitor BI2536 shows potential as a therapeutic option for Merkel cell carcinoma, as it shows high expression in the skin cancer.