C. Santos-Gallego, B. Picatoste, I. U. Njerve
Nov 25, 2014
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Journal
Circulation
Abstract
RISK pathway is the main cardioprotective pathway against ischemia-reperfusion (I-R) injury, and it starts with the phosphorylation (activation) of Akt. Phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a primary phosphatase that negatively regulates Akt phosphorylation. We hypothesized that the PTEN inhibitor, VO-OHpic, increases myocardial salvage, reduces myocardial infarction (MI) size, and mitigates left ventricular (LV) remodeling in a porcine model of I-R. Methods: Acute MI was induced in 10 pigs by balloon occlusion of the proximal LAD for 60 min, followed by reperfusion. Animals randomly received VO-OHpic 15 minutes prior to reperfusion, or saline for controls. Animals were evaluated with cardiac MRI and 3D-echo at 1 week and 1 month post MI. Histology was performed after 1 month to assess LV remodeling. Results: One week after MI, despite similar of myocardium at risk in both groups (40.9 ± 2.5% vs 40.9 ± 2.5%, p=NS), VO-OHpic significantly reduced MI size (28.3 ±1.7% vs 36.7 ± ...