J. Swader, B. Jacobson
1972
Citations
0
Influential Citations
50
Citations
Journal
Phytochemistry
Abstract
Abstract Acetazolamide (2-acetylamino-1,3,4-thiadiazole-5-sulfonamide), a compound commonly used to inhibit carbonic anhydrase has been ound to inhibit the photoreduction of 3-phosphoglyceric acid, NADP, and methyl viologen by isolated spinach chloroplasts. Acetazolamide had no effect on the photoreduction of methyl viologen by chloroplasts using ascorbate plus 2,6-dichloroindophenol, as an electron donor, indicating the site of the acetazolamide inhibition was not in photosystem I. There was no acetazolamide effect on NADP reduction by isolated chloroplasts using hydroxylamine instead of water as the electron donor. The results indicate that the site of acetazolamide inhibition is near the water-splitting side of photosystem II.