A. Lecklin, I. Lundell, Suvi Salmela
Aug 1, 2003
Citations
1
Influential Citations
45
Citations
Quality indicators
Journal
British Journal of Pharmacology
Abstract
The stimulatory effect of neuropeptide Y (NPY) on food intake is well established but the roles of the receptor subtypes Y1 and Y5 have been difficult to define. We have studied the effects of two novel Y1‐preferring and two Y5‐preferring agonists on feeding in guinea pigs. The Y1‐preferring receptor agonists [Arg6,Pro34]pNPY and [Phe7,Pro34]pNPY had high affinity for the Y1 receptor (Ki values 0.07 and 0.04 nM, respectively) and nanomolar affinity for the Y5 receptor. Administration of either compound into the third brain ventricle increased food intake equally to NPY. The Y5 agonist [Ala31,Aib32]pNPY displayed a moderate affinity for the Y5 receptor (Ki 7.42 nM) and a low affinity for Y1 (Ki 1.7 μM). This compound had only a modest effect on feeding. The other Y5‐preferring peptide [cPP1–7,NPY19–23,Ala31,Aib32,Gln34]hPP had a higher affinity at the Y5 receptor (Ki 1.32 nM) and also at the Y1 receptor (Ki 85 nM). It potently stimulated feeding: the food consumption after administration of this peptide was two‐fold compared to NPY. Our results support the view that both the receptor subtypes Y1 and Y5 are involved in the stimulation of feeding. As the action profiles of the Y1 and Y5 agonists on feeding parameters were different, it seems that they influence different phases of eating.