Epigenetic alterations associated with dexamethasone sodium phosphate through DNMT and TET in RPE cells

Paper

Epigenetic alterations associated with dexamethasone sodium phosphate through DNMT and TET in RPE cells

Published Nov 20, 2021 · Wenjie Liu, Sruthi Priya Mohan, N. Nagaraj

Molecular Vision

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2

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0

Influential Citations

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Abstract

Purpose To elucidate the mechanism behind epigenetic alteration associated with dexamethasone (DEX) sodium phosphate treatment. Methods We performed enzyme-linked immunosorbent assay to quantify changes in global DNA methylation and hydroxymethylation, quantitative real-time PCR (qRT-PCR) of the DNA methylation- and hydroxymethylation-related gene, in vitro DNA methyltransferase (DNMT) enzymatic activity assays with purified DNMTs, and DNA hydroxymethylation pattern with super-resolution imaging. Results We identified global DNA hypomethylation and hyper-hydroxymethylation upon DEX treatment, associated with aberrant mRNA expression levels of DNMT and ten-eleven translocation (TET) proteins. Additionally, DEX exposure could directly hinder DNMT activities. Conclusions We showed that DEX-induced epigenetic alterations are linked to aberrant DNMT and TET expression, potentially through an essential role of DNMT.

In Vitro Study

Rigorous Journal

Study Snapshot

Dexamethasone sodium phosphate treatment leads to epigenetic alterations in retinal cells, potentially through an essential role of DNMT.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Epigenetic alterations associated with dexamethasone sodium phosphate through DNMT and TET in RPE cells

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Citations

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