M. Matsui
Jun 5, 1986
Citations
0
Influential Citations
4
Citations
Journal
Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)
Abstract
In order to estimate the royal jelly content in so-called “Health Food” and crude drugs, 10-hydroxydecenoic acid (10-HDA), one of the specific fatty acids of royal jelly, was analyzed by gas chromatography (GC). 10-HDA in the royal jelly was determined by using an improved GC column packing material (OV-17 on Tenax GC) which does not require derivatization of 10-HDA to a volatile compound such as trimethylsilylate or methylate. This column was also applied to the analysis of fatty acids, hydroxy fatty acids and dibasic acids.10-HDA and 3-hydroxymyristic acid (internal standard) in the test samples were extracted with ethyl ether and determined directly on a gas chromatograph equipped with FID. The coefficient of variation for 10-HDA was 3.35% with a commerical royal jelly product sample. Confirmation was obtained by hydrogenation of 10-HDA to 10-hydroxydecanoic acid and reanalysis by GC.This method has been used to estimate the royal jelly content in Japanese and Chinese commercial royal jelly products by determination of 10-HDA.