Jiang Jian-wei
2013
Citations
0
Influential Citations
1
Citations
Quality indicators
Journal
Journal of Jinan University
Abstract
Aim:To investigate the inhibitory effect of Harmine hydrochloride on human pancreatic cancer cell line AsPC-1 and the possible mechanism.Methods: MTT and cell colony formation inhibitory assay were used to investigate the inhibition effect of Harmine hydrochloride on human pancreatic cancer cell line AsPC-1.Changes in apoptotic cell percentage were calculated by flow cytometry.Western blotting assay was used to determine cell apoptosis and autophagy.Results: MTT showed that Harmine hydrochloride could inhibited human pancreatic cancer AsPC-1cells in a dose-dependent manner.IC50 of Harmine hydrochloride on AsPC-1cells at 24 h,48 h and 72 h was 116.5 μmol·L-1,66 μmol·L-1,22.3 μmol·L-1 respectively;Cell clone formation inhibition assay demonstrated that cell clones were decreased with increase of the concentration of Harmine hydrochloride.Annexin V-FITC/PI staining also showed a dose-dependent effect on late apoptotic and necrotic cells.Western blotting assay showed that the apoptosis and autophagy landmark protein Caspase-3,PARP,LC3-II and P62 protein expression levels changed gradually with increasing of Harmine hydrochloride.Conclusion:Harmine hydrochloride inhibits the cell survival of human pancreatic cancer cell AsPC-1 in a dose-dependent manner.Harmine hydrochloride inhibits cell growth by inducing apoptotic and autophagy.