Hazardous Apoptotic Effects of 2-Bromopropane on Maturation of Mouse Oocytes, Fertilization, and Fetal Development

doi:10.3390/ijms11114361

Paper

Hazardous Apoptotic Effects of 2-Bromopropane on Maturation of Mouse Oocytes, Fertilization, and Fetal Development

Published Nov 3, 2010 · W. Chan

International Journal of Molecular Sciences

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6

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Abstract

2-Bromopropane (2-BP) is used as an alternative to ozone-depleting cleaning solvents. Previously, we reported that 2-BP has cytotoxic effects on mouse blastocysts and is associated with defects in subsequent development. Here, we further investigate the effects of 2-BP on oocyte maturation and subsequent pre- and post-implantation development, both in vitro and in vivo. Notably, 2-BP induced a significant reduction in the rates of oocyte maturation, fertilization, and in vitro embryonic development. Treatment of oocytes with 2-BP during in vitro maturation (IVM) resulted in increased resorption of postimplantation embryos and decreased fetal weights. Experiments with a mouse model disclosed that consumption of drinking water containing 20 μM 2-BP led to decreased oocyte maturation in vivo and fertilization in vitro, as well as impairment of early embryonic development. Interestingly, pretreatment with a caspase-3-specific inhibitor effectively prevented 2-BP-triggered hazardous effects, suggesting that embryonic impairment by 2-BP occurs via a caspase-dependent apoptotic process. A study using embryonic stem cells as the assay model conclusively demonstrated that 2-BP induces cell death processes through apoptosis and not necrosis, and inhibits early embryo development in mouse embryonic stem cells. These results collectively confirm the hazardous effects of 2-BP on embryos derived from pretreated oocytes.

In Vitro Study

Study Snapshot

2-Bromopropane reduces oocyte maturation, fertilization, and embryonic development in mice, with apoptosis being the main mechanism of embryo impairment.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Hazardous Apoptotic Effects of 2-Bromopropane on Maturation of Mouse Oocytes, Fertilization, and Fetal Development

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References

Citations

Prevention of ochratoxin A‐induced oxidative stress‐mediated apoptotic processes and impairment of embryonic development in mouse blastocysts by liquiritigenin

Liquiritigenin effectively protects against ochratoxin A-induced apoptosis and embryotoxicity in mouse blastocysts, preventing impairment of preimplantation and postimplantation embryonic development and fetal weight loss.

Protective Effects of Liquiritigenin against Citrinin-Triggered, Oxidative-Stress-Mediated Apoptosis and Disruption of Embryonic Development in Mouse Blastocysts

Liquiritigenin effectively prevents apoptosis and disrupts embryonic development in mouse blastocysts when exposed to the mycotoxin citrinin.

Resveratrol protects against methylglyoxal‐induced apoptosis and disruption of embryonic development in mouse blastocysts

Resveratrol protects against methylglyoxal-induced apoptosis and disruption of embryonic development in mouse blastocysts, preventing toxicity and promoting implantation.

Resveratrol protects against hazardous effects of 2- bromopropane on maturation of mouse oocytes, fertilization and fetal development

Resveratrol protects against the harmful effects of 2-bromopropane on oocyte maturation, fertilization, and embryonic development in mice.

Resveratrol Protects against 2-Bromopropane-Induced Apoptosis and Disruption of Embryonic Development in Blastocysts

Resveratrol protects against 2-Bromopropane-induced apoptosis and disrupts embryonic development in mouse blastocysts, preventing toxicity and promoting embryonic development.