S. Narita, T. Kitagawa
Mar 25, 1989
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0
Influential Citations
11
Citations
Journal
Chemical & Pharmaceutical Bulletin
Abstract
2-(p-Aminomethylphenyl)-N, N-dimethyl-2H-benzotriazolyl-5-amine (1) and two related compounds (2 and 3) were synthesized as highly sensitive fluorescence derivatization reagents for carboxylic acids. These reagents reacted with carboxylic acids activated with 2-bromo-1-ethylpyridinium tetrafluoroborate to produce the corresponding fluorescent amides at room temperature. We investigated the fluorescence characteristics and the chromatographic behavior of the benzamide derivatives of the reagents. Compound 1 was selected for use in high-performance liquid chromatography (HPLC). A highly sensitive and convenient fluorescence HPLC method was developed for the determination of carboxylic acids. The detection limit for carboxylic acids was 15 fmol per injection.