Assay of succinate dehydrogenase activity by a colorimetric-continuous method using iodonitrotetrazolium chloride as electron acceptor.

doi:10.1006/ABIO.1993.1360

Paper

Assay of succinate dehydrogenase activity by a colorimetric-continuous method using iodonitrotetrazolium chloride as electron acceptor.

Published Aug 1, 1993 · P. Munujos, Jaume Coll-Cantí, F. González-Sastre

Analytical biochemistry

Q3 SJR score

128

Citations

6

Influential Citations

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Abstract

A spectrophotometric assay method for determining succinate dehydrogenase activity is described in which iodonitrotetrazolium chloride is used as a final electron acceptor. The enzyme activity is determined by measuring the formation of formazan due to the tetrazolium salt reduction. The assay is continuous, rapid, simple, and sensitive, and may be used in the determination of enzyme activity either in tissue homogenates or as a marker of the mitochondrial fraction in cell fractionation procedures.

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Study Snapshot

The spectrophotometric assay method using iodonitrotetrazolium chloride is a rapid, simple, and sensitive method for determining succinate dehydrogenase activity in tissue homogenates and as a marker for mitochondrial fractions in cell fractionation procedures.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Assay of succinate dehydrogenase activity by a colorimetric-continuous method using iodonitrotetrazolium chloride as electron acceptor.

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References

Citations

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