Binding of human single-stranded DNA binding protein to DNA damaged by the anticancer drug cis-diamminedichloroplatinum (II).

Paper

Binding of human single-stranded DNA binding protein to DNA damaged by the anticancer drug cis-diamminedichloroplatinum (II).

Published Nov 15, 1992 · Carol K. Clugston, K. McLaughlin, Mark K. Kenny

Cancer research

Q1 SJR score

89

Citations

1

Influential Citations

Semantic Scholar

Abstract

The chemotherapeutic drug cis-diamminedichloroplatinum (II) covalently binds to DNA resulting in a variety of adducts and cross-links which are thought to be responsible for the toxicity of the drug. We have used the gel mobility shift assay to detect proteins which bind to DNA treated in vitro with cis-diamminedichloroplatinum (II) and have identified two complexes which bind with increased affinity to cis-diamminedichloroplatinum (II)-damaged DNA. Using monoclonal antibodies we have shown that one complex, B1, contains human single-stranded DNA binding protein, a protein known to be involved in the in vitro repair synthesis assay of mammalian excision repair.

In Vitro Study

Highly Cited

Study Snapshot

Human single-stranded DNA binding protein (B1) binds with increased affinity to DNA damaged by the anticancer drug cis-diamminedichloroplatinum (II), suggesting its potential role in mammalian excision repair.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Binding of human single-stranded DNA binding protein to DNA damaged by the anticancer drug cis-diamminedichloroplatinum (II).

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