D. Charon, R. Sarfati, D. R. Strobach
Dec 1, 1969
Citations
0
Influential Citations
19
Citations
Journal
European journal of biochemistry
Abstract
3-Deoxy d-manno-octulosonic acid has been synthesised starting from d-mannose via 2-deoxy d-manno heptose, cyanohydrine addition, and selective oxidation of the epimeric hydroxyl group of the resulting 3-deoxy octonic acids. When treated with periodate in 0.1 N sulphuric acid solution at 0°, the deoxy octulosonic acid and other 3-deoxy aldulosonic acids all yield one molar equivalent of β-formyl pyruvate. Conditions are described in which β-formyl pyruvate can be estimated by the thiobarbituric acid method; hence, when treated successively with periodate and thiobarbiturate, all 3-deoxy aldulosonic acids have the same molar extinction coefficient [(92 ± 5)103 in dilute acid; 133 × 103 in cyclohexanone] and can be estimated by this method without the use of standards.