Cinnamyl alcohol attenuates vasoconstriction by activation of K+ channels via NO-cGMP-protein kinase G pathway and inhibition of Rho-kinase

doi:10.3858/emm.2012.44.12.083

Paper

Cinnamyl alcohol attenuates vasoconstriction by activation of K+ channels via NO-cGMP-protein kinase G pathway and inhibition of Rho-kinase

Published Nov 26, 2012 · Y. Kang, In-Jun Yang, K. Morgan

Experimental & Molecular Medicine

Q1 SJR score

15

Citations

1

Influential Citations

Full textSemantic Scholar

Abstract

Cinnamyl alcohol (CAL) is known as an antipyretic, and a recent study showed its vasodilatory activity without explaining the mechanism. Here we demonstrate the vasodilatory effect and the mechanism of action of CAL in rat thoracic aorta. The change of tension in aortic strips treated with CAL was measured in an organ bath system. In addition, vascular strips or human umbilical vein endothelial cells (HUVECs) were used for biochemical experiments such as Western blot and nitrite and cyclic guanosine monophosphate (cGMP) measurements. CAL attenuated the vasoconstriction of phenylephrine (PE, 1 µM)-precontracted aortic strips in an endothelium-dependent manner. CAL-induced vasorelaxation was inhibited by pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 10-4 M), methylene blue (MB; 10-5 M) and 1 H-[1,2,4]-oxadiazolole-[4,3-a] quinoxalin-10one, (ODQ; 10-6 or 10-7 M) in the endothelium-intact aortic strips. Atrial natriuretic peptide (ANP; 10-8 or 10-9 M) did not affect the vasodilatory effect of CAL. The phosphorylation of endothelial nitric oxide synthase (eNOS) and generation of nitric oxide (NO) were stimulated by CAL treatment in HUVECs and inhibited by treatment with L-NAME. In addition, cGMP and PKG1 activation in aortic strips treated with CAL were also significantly inhibited by L-NAME. Furthermore, CAL relaxed Rho-kinase activator calpeptin-precontracted aortic strips, and the vasodilatory effect of CAL was inhibited by the ATP-sensitive K+ channel inhibitor glibenclamide (Gli; 10-5 M) and the voltage-dependent K+ channel inhibitor 4-aminopyridine (4-AP; 2 × 10-4 M). These results suggest that CAL induces vasorelaxation by activating K+ channels via the NO-cGMP-PKG pathway and the inhibition of Rho-kinase.

In Vitro Study

Study Snapshot

Cinnamyl alcohol induces vasorelaxation by activating K+ channels through the NO-cGMP-PKG pathway and inhibiting Rho-kinase.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Sign up to use Study Snapshot

Consensus is limited without an account. Create an account or sign in to get more searches and use the Study Snapshot.

Create an account

Paper

Cinnamyl alcohol attenuates vasoconstriction by activation of K+ channels via NO-cGMP-protein kinase G pathway and inhibition of Rho-kinase

Sign up to use Study Snapshot

References

Antiproliferative Activity of Cinnamomum cassia Constituents and Effects of Pifithrin-Alpha on Their Apoptotic Signaling Pathways in Hep G2 Cells

Cinnamaldehyde, the most potent antiproliferative component of Cinnamomum cassia, induces apoptosis in Hep G2 cells through p53 induction and CD95 (APO-1) signaling pathways.

Activation of cGMP-Dependent Protein Kinase Stimulates Cardiac ATP-Sensitive Potassium Channels via a ROS/Calmodulin/CaMKII Signaling Cascade

PKG plays a dual role in cardiac KATP channels, regulating cardiomyocyte excitability and contributing to cardiac protection against ischemia-reperfusion injury through a ROS/calmodulin/CaMKII signaling pathway.

Heme-Dependent and Independent Soluble Guanylate Cyclase Activators and Vasodilation

Heme-independent soluble guanylate cyclase activators show potential for treating diseases associated with endothelial dysfunction, but are currently in clinical trials.

Endothelium-derived Vasoactive Factors and Hypertension: Possible Roles in Pathogenesis and as Treatment Targets

Endothelial dysfunction and hypertension are linked to various endothelial pathways, which could be targeted for novel treatments.

Mechanisms of nitrite reduction to nitric oxide in the heart and vessel wall.

Nitrite-mediated nitric oxide formation plays critical physiological and pathological roles in the heart and vessels, and is influenced by oxygen tension, pH, reducing substrates, and nitrite levels.

Citations

Calpain Inhibitor Calpeptin Alleviates Ischemia/Reperfusion-Induced Acute Kidney Injury via Suppressing AIM2 Inflammasome and Upregulating Klotho Protein

Calpeptin improves kidney function and reduces damage in ischemia/reperfusion-induced acute kidney injury by suppressing AIM2 inflammasome activation and increasing Klotho protein levels.

Hypoglycemia increases endothelial-dependent vasodilation through suppressing phosphorylation at Threonine 495/497 site of endothelial nitric oxide synthase.

Hypoglycemia increases endothelial-dependent vasodilation by suppressing eNOS phosphorylation, leading to a beneficial cardiovascular effect in rats.

Antiviral effects of Ma Huang Tang against H1N1 influenza virus infection in vitro and in an ICR pneumonia mouse model.

Ma Huang Tang (MHT) effectively reduces influenza virus A pneumonia in mice, likely due to its antiviral activity and its ability to regulate immune function and the MyD88-dependent signaling pathway of TLR4.

Effects of Qibaipingfei capsules on pulmonary vascular relaxation through KATP channel activation by the NO/cGMP signaling pathway.

Qibaipingfei capsules induce endothelial-dependent relaxation in pulmonary artery rings through activation of the KATP channel in smooth muscles through the NO-cGMP-dependent pathway.

Cardiovascular Activity of the Chemical Constituents of Essential Oils

Essential oil chemical constituents show potential for treating cardiovascular diseases, with their mechanisms of action being studied in ongoing research.

Age and harvest season affect the phenylpropenoid content in cultivated European Rhodiola rosea L.

Rhizomes of Rhodiola rosea L. have higher phenylpropenoid content when harvested in March, with a decrease from year 3 to year 5, and a 5-30% aglycon cinnamyl alcohol content depending on plant origin.

Cardiovascular and renal effect of CNAAC: An innovatively designed natriuretic peptide.

CNAAC, a novel natriuretic peptide analogue, has a potent vasodilating effect and diuretic and hypotensive effects, likely through activating K(+) channels via the NPR-A/sGC/cGMP pathway.

Simultaneous determination of cinnamaldehyde and its metabolite in rat tissues by gas chromatography-mass spectrometry.

This study developed a selective and sensitive method for simultaneously determining cinnamaldehyde and its metabolite cinnamyl alcohol in rat tissues, providing accurate data for tissue study.