A. Keightley, M. Dobrzyńska, M. Podbielkowska
1996
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Abstract
Coumarin and five derivatives (4-hydroxycoumarin, 7-hydroxycoumarin, 7-methylcoumarin, 7-methoxycoumarin, and 7-methoxy-4-methylcoumarin) were investigated for their potential as antimitotic agents. Coumarin and 4-hydroxycoumarin completely inhibited mitosis yet did not induce chromosomal aberrations in Allium sativum promeristem after 24 h treatment, and when postincubated in water did not exhibit mitotic recovery. 7-hydroxycoumarin also strongly inhibited cell proliferation (77% mitotic inhibition after 24 h). Sharp declines in mitotic activity of A. sativum promeristem were noted for 7-methylcoumarin, 7-methoxycoumarin, and 7-methoxy-4-methylcoumarin (83%, 90%, and 64% mitotic inhibition, respectively) in 24 h incubations. Chromosomal aberrations associated with 7-hydroxycoumarin treatment included shortening and thickening of chromosomes, postponed chromatid separation, anaphase and telophase bridges, and irregularly shaped nuclei. Disruption of normal chromosomal structure was also observed after exp...