J. Roth, D. B. Mccormick, L. D. Wright
Dec 10, 1970
Citations
0
Influential Citations
10
Citations
Journal
The Journal of biological chemistry
Abstract
Abstract A culture medium, which contains biotin l-sulfoxide as the sole substrate, does not support the growth of a soil pseudomonad that is known to utilize biotin as a sole source of carbon, nitrogen, and sulfur. Both the relative rate and extent of transport of 14C-biotin and 14C-biotin analogues by 0.9% NaCl solution-washed cells grown in medium containing biotin as the only substrate are indicated by the following series: biotin sulfone ≥ biotin l-sulfoxide g biotin d-sulfoxide g biotin g dethiobiotin. These results indicate that transport of l-sulfoxide into the cell is not the process that limits the rate of growth of the pseudomonad. A culture medium containing 60% of carbonyl-labeled biotin l-sulfoxide and 40% of unlabeled biotin supports the growth of the bacteria until all of the biotin in the medium is utilized. On depletion of biotin, radioactivity loss ceases and a new 14C-metabolite appears. On the 16th day of incubation, approximately 25% of the remaining material, which is sensitive to the color-producing p-dimethylaminocinnamaldehyde, is the metabolite. The structure of the unknown metabolite, determined by infrared, proton magnetic resonance, mass spectroscopy, and by elemental and other analyses, is β-hydroxybiotin l-sulfoxide.