W. Im, J. Roth, D. B. Mccormick
Dec 10, 1970
Citations
0
Influential Citations
11
Citations
Journal
The Journal of biological chemistry
Abstract
Abstract Biotin d-sulfoxide can be utilized as a sole source of carbon, nitrogen, and sulfur for growth of a pseudomonad. The following series indicates the relative rates of uptake of 14C-biotin and the 14C-analogues by 0.9% NaCl solution-washed cells, which were grown in a medium containing biotin d-sulfoxide: biotin sulfone g biotin l-sulfoxide g biotin d-sulfoxide g biotin g dethiobiotin. Bacteria grown on a salts-trace element medium containing 0.3% 14C-carbonyl-labeled biotin d-sulfoxide have an extended initial period of slow growth as compared to that on a medium containing 14C-biotin. Biotin is slowly produced in the d-sulfoxide culture, and the concentration of biotin reaches a maximum of approximately 5 to 7% of the total remaining substrate during the log phase of growth. Several metabolites have been isolated from culture filtrates of the pseudomonad grown for 9 days on a medium containing 14C-carbonyl-labeled d-sulfoxide. Purification of the following metabolites has been achieved by anion exchange chromatography over Dowex 1-X2: biotin; bisnorbiotin; tetranorbiotin; biotin l-sulfoxide; biotin sulfone; and a new catabolite, bisnorbiotin sulfone. The results are consistent with the conclusion that reduction of d-sulfoxide to the thioether precedes utilization of the sulfur atom. Thus, the major pathway for the degradation of biotin d-sulfoxide appears to be through biotin.