S. K. Lau, Fabian Zaccardo, Michael J. Little
Jun 5, 1998
Citations
2
Influential Citations
58
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The ability of 5-carboxyfluorescein succinimidyl ester (CFSE) to derivatize amino acids at very low concentrations has been investigated. CFSE can derivatize nanomolar concentrations of amino acids, which represents a 1000-fold improvement compared to fluorescein isothiocyanate (FITC). Although the reactive probe is not fluorogenic, few hydrolysis products result from derivatization, leading to relatively clean electropherograms compared to FITC derivatization. Derivatizations of γ-aminobutyric acid suggest that CFSE may be a practical amine-reactive fluorescent probe for the quantification of neurotransmitters in microdialysates.