C. Xiao
2009
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0
Influential Citations
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Citations
Quality indicators
Journal
Chinese Journal of Analytical Chemistry
Abstract
A HPLC method was established for the determination of 3-hydroxy-3-methylglutaryl coenzyme A(HMG-CoA) reductase inhibitor activity in vitro by measuring the concentration of β-nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt(NADPH) in the system.The chromatographic conditions were as follows:Hypersil C18 column;phosphate liquor(pH 7.2) as mobile phase at a flow rate of 1 mL/min,column temperature of 25 ℃,detection wavelength of 337 nm.An excellent linearity over the range of 0.5-100 μmol/L(r=0.9995) was observed.The detection limit was 0.01 μmol/L,and the recovery rate of β-nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt(NADPH) was 99.8%-100.7% with a relative standard deviation(RSD) of 1.0%-1.3%(n=5).